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Anti-MMP12 Antibody EP1261Y
Also for MMP12 (NM_002426)
|A synthetic peptide corresponding to residues on the C-terminus of human MMP-12 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:30; FC: 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens matrix metallopeptidase 12 (MMP12)|
|HME; ME; MME; MMP-12|
Entrez Gene 4321 Human
Entrez Gene 17381 Mouse
Entrez Gene 117033 Rat
|Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3). |
|ProteaseDruggable Genome |
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Western blot - MMP12 antibody [EP1261Y] - Carboxyterminal end; .Predicted band size : 54 kDa.
Immunohistochemistry (Paraffin-embedded sections) - MMP12 antibody [EP1261Y]; Ab52897 (1:100) staining human MMP12 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - MMP12 antibody [EP1261Y]; Immunofluorescent staining of HeLa cells using TA300810 (1:100).
Flow Cytometry - MMP12 antibody [EP1261Y] - Carboxyterminal end; Overlay histogram showing A549 cells stained with TA300810 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.