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Home Antibody All anti-ITGAL antibodies

Anti-ITGAL Antibody EP1285Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300808
  • Rabbit Monoclonal Antibody against ITGAL (Clone EP1285Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400809) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
FC(1)
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Also for ITGAL (NM_002209)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human LFA-1a was used as an immunogen.
Clone NameEP1285Y IsotypeIgG
Species ReactivityHuman, Cynomolgus Monkey (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IP: 1:100; FC: 1:80 - 1:100; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; Blocking: Use at an assay dependent concentration
Predicted MW Explanation 129 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL), transcript variant 1
Alternative NameCD11A; LFA-1; LFA1A
Database LinkNP_002200
Entrez Gene 3683 Human
Entrez Gene 711945 Monkey
FunctionIn the immune system, integrins have essential roles in leukocyte trafficking and function. These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signaling. JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor, has been identified as a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections (3)
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Natural killer cell mediated cytotoxicityLeukocyte transendothelial migrationRegulation of actin cytoskeletonViral myocarditis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CD11a antibody [EP1285Y]; Anti-CD11a antibody [EP1285Y] at 1/20000 dilution + Jurkat cell lysate at 10 ug/ml.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 129 kDa.Observed band size : 180 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - CD11a antibody [EP1285Y]; Immunohistochemical staining of paraffin embedded human spleen tissue using TA300808 at 1/100 dilution.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-CD11a antibody [EP1285Y]; Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with TA300808 at 1/50 dilution.
FC Image
Flow Cytometry-CD11a antibody [EP1285Y](TA300808); Overlay histogram showing Jurkat cells stained with TA300808 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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