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Home Antibody All anti-SMAD3 antibodies

Anti-SMAD3 Antibody EP823Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300787
  • Rabbit Monoclonal Antibody against SMAD3 (Clone EP823Y) (Phospho-specific)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401784) , 20ug
100ul 379 In Stock
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WB(3)
IHC(1)
IF(2)
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Also for SMAD3 (NM_005902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-specific peptide corresponding to residues surrounding Ser423 and Ser425 of human Smad3 was used as an immunogen. The antibody only detects Smad3 phosphorylated on Serine 423 and Serine 425.
Clone NameEP823Y IsotypeTissue culture supernatant
Species ReactivityHuman, Mouse(WB only) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2000; ICC/IF: 1:100 - 1:250; ELISA: Use at an assay dependent concentration; IHC-Fr: Use at an assay dependent concentration; IHC-P: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for Flow Cyt or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens SMAD family member 3 (SMAD3), transcript variant 1
Alternative NameHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Database LinkNP_005893
FunctionSmad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-ß/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-ß), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-ß receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- ß target genes. Interaction of Smad3 with Akt, can initiate TGF- ß induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis
Related Pathway
Adherens junction
Adipogenesis
Cell cycle
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody; .Predicted band size : 48 kDa.</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
WB Image
Western blot - Smad3 antibody [EP823Y]; All lanes : Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody at 1/2000 dilution.Lane 1 : (A) HL-60 cell lysates at 10ug untreated.Lane 2 : (B) HL-60 cell lysates at 10ug treated with TGF.Predicted band size : 48 kDa.Observed band size : 55 kDa .Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
WB Image
Western blot - Smad3 (phospho S423 + S425) antibody [EP823Y]; All lanes : Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody at 1/1000 dilution.Lane 1 : Lysate prepared from untreated human A549 cells.Lane 2 : Lysate prepared from untreated human A549 cells for 30min.Lane 3 : Lysate prepared from TGF-?1 cells at 10ng/ml for 30min.Lane 4 : Lysate prepared from TNF-a cells at 20ng/ml for 30min.Lane 5 : Lysate prepared from TGF-?1 and TNF-a cells at above doses for 30min.Lane 6 : Blank DMEM media.Lysates/proteins at 20 µg per lane.Secondary.Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 48 kDa.Observed band size : 48 kDa.Exposure time : 1 hourThis image is a courtesy of Aaron Gardner</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
IHC Image
Immunohistochemistry - Smad3 (phospho S423 + S425) antibody [EP823Y]; Immunohistochemical analysis of Smad3 in paraffin embedded human liver carcinoma tissue using TA300787 at 1/100 dilution.</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
IF Image
Immunocytochemistry/ Immunofluorescence - Smad3 antibody [EP823Y]; Immunofluorescent staining of Smad3 in HeLa cells using TA300787 at 1/100 dilution.</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
IF Image
Immunocytochemistry/ Immunofluorescence - Smad3 (phospho S423 + S425) antibody [EP823Y]; TA300787 stainingSmad3 (phospho S423 + S425) inhuman TII Pneumocyte A549c ells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde andpermeabilized with 0.1%Triton x100before blocking with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/200: in 3% BSA in 1x PBST) for24 hours at 4°C. A TRITC-conjugatedgoat polyclonal torabbit IgG was used as secondary antibody at 1/200 dilution.</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>

 

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