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Anti-SMAD3 Antibody EP823Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300787 Rabbit Monoclonal Antibody against SMAD3 (Clone EP823Y) (Phospho-specific) 100ul $379 In Stock
LC401784 SMAD3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic phospho-specific peptide corresponding to residues surrounding Ser423 and Ser425 of human Smad3 was used as an immunogen. The antibody only detects Smad3 phosphorylated on Serine 423 and Serine 425.
Clone NameEP823Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2000; ICC/IF: 1:100 - 1:250; ELISA: Use at an assay dependent concentration; IHC-Fr: Use at an assay dependent concentration; IHC-P: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens SMAD family member 3 (SMAD3), transcript variant 1
Alternative NameHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Database LinkNP_005893
Entrez Gene 4088 Human
Entrez Gene 17127 Mouse
FunctionSmad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-ß/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-ß), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-ß receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- ß target genes. Interaction of Smad3 with Akt, can initiate TGF- ß induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody; .Predicted band size : 48 kDa.

WB Image
Western blot - Smad3 antibody [EP823Y]; All lanes : Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody at 1/2000 dilution.Lane 1 : (A) HL-60 cell lysates at 10ug untreated.Lane 2 : (B) HL-60 cell lysates at 10ug treated with TGF.Predicted band size : 48 kDa.Observed band size : 55 kDa .Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

WB Image
Western blot - Smad3 (phospho S423 + S425) antibody; All lanes : Anti-Smad3 (phospho S423 + S425)antibody at 1/1000 dilution. Lane 1 : Untreated Human A549 cells lysate.Lane 2 : Untreated Human A549 cells lysate for 30min.Lane 3 : TGF-ß1 cells lysate at 10ng/ml for 30min.Lane 4 : TGF-a cells lysate at 20ng/ml for 30min.Lane 5 : TGF-ß1 and TNF-a cell lysat at above doses for 30min.Lane 6 : Blank DMEM media.Lysates/proteins at 20 ug per lane.

IHC Image
Immunohistochemistry - Smad3 (phospho S423 + S425) antibody [EP823Y]; Immunohistochemical analysis of Smad3 in paraffin embedded human liver carcinoma tissue using TA300787 at 1/100 dilution.

IF Image
Immunocytochemistry/ Immunofluorescence - Smad3 antibody [EP823Y]; Immunofluorescent staining of Smad3 in HeLa cells using TA300787 at 1/100 dilution.

IF Image
ICC/IF - Smad3 (phospho S423 + S425) antibody; TA300787 stainingSmad3 (phospho S423 + S425) inhuman TII Pneumocyte A549c ells by ICC/IF. Cells were fixed with paraformaldehyde andpermeabilized with 0.1%Triton x100before blocking with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/200: in 3% BSA in 1x PBST) for24 hours at 4°C. A TRITC-conjugatedgoat polyclonal torabbit IgG was used as secondary antibody at 1/200 dilution.


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