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Anti-TUBA1B Antibody EP1332Y
Also for TUBA1B (NM_006082)
|A synthetic peptide corresponding to residues near the N-term of human a-Tubulin was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:250 - 1:500; IHC - Wholemount: Use at an assay dependent concentration; WB: 1:1000 - 1:50000; IP: 1:50; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens tubulin, alpha 1b (TUBA1B)|
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is pos-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in human (4). |
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Western blot - alpha Tubulin antibody [EP1332Y]; Anti-alpha Tubulin [EP1332Y] antibody at 1/50000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Paraffin-embedded sections) - alpha Tubulin antibody [EP1332Y]; Ab52866 (1:250) staining human alpha Tubulin in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunohistochemistry (Frozen sections) - Anti-alpha Tubulin antibody [EP1332Y]; Immunohistochemical analysis of rat kidney tubule tissue, staining alpha Tubulin with ab52866.
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [EP1332Y]; ab52866 staining alpha Tubulin in 293 Human embryonic kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 2 hours at 23°C. Samples were incubated with primary antibody (1/200 in 0.5% saponin) for 2 hours at 23°C. An Alexa Fluor?555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
Flow Cytometry-alpha Tubulin antibody [EP1332Y](ab52866); Overlay histogram showing HeLa cells stained with ab52866 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.