OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-TUBA1B antibodies

Anti-TUBA1B Antibody EP1332Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300785
  • Rabbit Monoclonal Antibody against TUBA1B (Clone EP1332Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401831) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(2)
IF(1)
FC(1)
spacer
Also for TUBA1B (NM_006082)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-term of human a-Tubulin was used as immunogen.
Clone NameEP1332Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Pig, Fruit fly (Drosophila melanogaster) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:250 - 1:500; IHC - Wholemount: Use at an assay dependent concentration; WB: 1:1000 - 1:50000; IP: 1:50; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution; IHC-Fr: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens tubulin, alpha 1b (TUBA1B)
Alternative NameK-ALPHA-1
Database LinkNP_006073
Entrez Gene 10376 Human
Entrez Gene 22143 Mouse
Entrez Gene 500929 Rat
FunctionMicrotubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is pos-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in human (4).
Related PathwayDruggable Genome Gap junctionPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - alpha Tubulin antibody [EP1332Y]; Anti-alpha Tubulin [EP1332Y] antibody at 1/50000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - alpha Tubulin antibody [EP1332Y]; Ab52866 (1:250) staining human alpha Tubulin in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-alpha Tubulin antibody [EP1332Y]; Immunohistochemical analysis of rat kidney tubule tissue, staining alpha Tubulin with TA300785.
IF Image
Immunocytochemistry/ Immunofluorescence - alpha Tubulin antibody [EP1332Y]; TA300785 staining alpha Tubulin in 293 Human embryonic kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 2 hours at 23°C. Samples were incubated with primary antibody (1/200 in 0.5% saponin) for 2 hours at 23°C. An Alexa Fluor?555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
FC Image
Flow Cytometry-alpha Tubulin antibody [EP1332Y](TA300785); Overlay histogram showing HeLa cells stained with TA300785 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title