Home Antibody All anti-TUBA1B antibodies
Anti-TUBA1B Antibody EP1332Y
Also for TUBA1B (NM_006082)
|A synthetic peptide corresponding to residues near the N-term of human a-Tubulin was used as immunogen.|
|Mouse, Rat, Human, Pig, Fruit fly (Drosophila melanogaster)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:250 - 1:500; IHC - Wholemount: Use at an assay dependent concentration; WB: 1:1000 - 1:50000; IP: 1:50; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens tubulin, alpha 1b (TUBA1B)|
Entrez Gene 10376 Human
Entrez Gene 22143 Mouse
Entrez Gene 500929 Rat
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is pos-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in human (4). |
|Druggable Genome Gap junctionPathogenic Escherichia coli infection|
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - alpha Tubulin antibody [EP1332Y]; Anti-alpha Tubulin [EP1332Y] antibody at 1/50000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Paraffin-embedded sections) - alpha Tubulin antibody [EP1332Y]; Ab52866 (1:250) staining human alpha Tubulin in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunohistochemistry (Frozen sections) - Anti-alpha Tubulin antibody [EP1332Y]; Immunohistochemical analysis of rat kidney tubule tissue, staining alpha Tubulin with TA300785.
TA300785 staining alpha Tubulin in 293 Human embryonic kidney cells by ICC/IF (ICC/IF). Cells were fixed with paraformaldehyde and blocked with 10% serum for 2 hours at 23°C. Samples were incubated with primary antibody (1/200 in 0.5% saponin) for 2 hours at 23°C. An Alexa Fluor 555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
Flow Cytometry-alpha Tubulin antibody(TA300785); Overlay histogram showing HeLa cells stained with TA300785 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.