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Anti-EPS15L1 Antibody EP1145Y
Also for EPS15L1 (NM_021235)
|A synthetic peptide corresponding to residues near the C-terminus of human EPS15R was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000; IP: 1:70; ICC: 1:50 - 1:100; FC: 1:10; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens epidermal growth factor receptor pathway substrate 15-like 1 (EPS15L1), transcript variant 2|
Entrez Gene 58513 Human
|EPS15 and EPS15R are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Evidence suggests that eps15 and eps15R are involved in endocytosis (1). The most striking difference between these two related proteins is that Eps15R is also found in the nucleus, whereas Eps15 is excluded from this compartment at steady state (2). Eps15R plays a key role in clathrin-mediated endocytosis of transmembrane receptors. Using a mutational approach, it has been found that the second ubiquitin-interacting motif (UIM) of Eps15 and Eps15R is essential for their ubiquitination (3). |
|Druggable Genome |
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Western blot - EPS15R antibody [EP1145Y]; Anti-EPS15R antibody [EP1145Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 94 kDa.Observed band size : 125 kDa .
Immunohistochemistry (Paraffin-embedded sections) - EPS15R antibody [EP1145Y]; TA300766, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.