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Home Antibody All anti-ACLY antibodies

Anti-ACLY Antibody EP828Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300735
  • Rabbit Monoclonal Antibody against ACLY (Clone EP828Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420122) , 20ug
100ul 325 3-7 Days
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WB(1)
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Also for ACLY (NM_001096)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human ATP citrate lyase was used as immunogen.
Clone NameEP828Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:5000; IP: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IHC-P.

Reference Data

Target NameHomo sapiens ATP citrate lyase (ACLY), transcript variant 1
Alternative NameACL; ATPCL; CLATP
Database LinkNP_001087
FunctionATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).
Related Pathway

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WB Image
Western blot - ATP citrate lyase (phospho S455) antibody [EP828Y]; All lanes : Anti-ATP citrate lyase (phospho S455) antibody [EP828Y] at 1/5000 dilution.Lane 1 : Untreated 3T3 cell lysates.Lane 2 : Insulin treated 3T3 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 125 kDa .

 

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