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Anti-NRAS Antibody EP1125Y
Also for NRAS (NM_002524)
| A synthetic peptide corresponding to residues near the N-terminal region of human Ras was used as an immunogen. Predicted to react with H-Ras and K-Ras.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500,000; FC: 1: 100; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS)|
|ALPS4; N-ras; NRAS1; NS6|
|Ras (p21), a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4). |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - Ras antibody [EP1125Y]; Anti-Ras antibody [EP1125Y] at 1/500000 dilution + C6 cell lysate at 10 µg/ml.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 18 kDa.Observed band size : 18 kDa.
Western blot ; Anti-Ras antibody [EP1125Y] at 1/500 dilution + Human Ras full length protein at 0.01 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
Flow Cytometry - Ras antibody [EP1125Y]; TA300732 at 1/100 dilution staining Ras in permeabilized PC-12 cells by flow cytometry (red). Rabbit IgG negative control (green).