Home Antibody All anti-TUBB antibodies
Also for TUBB (NM_178014)
|A synthetic peptide corresponding to residues near the C-term of human B-Tubulin was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC-P: 1:100 - 1:250; WB: 1:1000 - 1:2000; IP: 1:50; ICC/IF: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Protein A purified (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens tubulin, beta class I (TUBB)|
|M40; OK/SW-cl.56; TUBB1; TUBB5|
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3). |
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Western blot - Anti-beta Tubulin antibody; Anti-beta Tubulin antibody at 1/1000 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 49 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody; Immunohistochemical analysis of paraffin-embedded Human muscle tissue labelling beta Tubulin with ab134185 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody; Immunofluorescent analysis of HeLa cells labelling beta Tubulin with ab134185 at 1/50 dilution.