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Anti-MAP3K5 Antibody EP553Y
Also for MAP3K5 (NM_005923)
|A synthetic peptide corresponding to residues in N-terminus of human MEKK5/ASK1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:100
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)|
|ASK1; MAPKKK5; MEKK5|
|Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k type protein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK) family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5 phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylates MEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF via TRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1). ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threonine phosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, a catalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis. Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, such as in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has been implicated as a therapeutic target for neurodegenerative disorder sand cardiac dysfunction (4) |
MAPK signaling pathway
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Western blot - ASK1 antibody [EP553Y]; Anti-ASK1 antibody [EP553Y] at 1/2000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit, HRP labelled at 1/2000 dilution.Predicted band size : 155 kDa.Observed band size : 155 kDa.
Immunohistochemistry (Paraffin-embedded sections) - ASK1 antibody [EP553Y]; Ab45178 (1/10) staining human ASK1 in human lung carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - ASK1 antibody [EP553Y]; Immunofluorescent staining of HeLa cells using ab45178 (1/10).
Flow Cytometry - ASK1 antibody [EP553Y]; Overlay histogram showing HeLa cells stained with ab45178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.