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Home Antibody All anti-ADD1 antibodies

Anti-ADD1 Antibody EP733Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300679
  • Rabbit Monoclonal Antibody against ADD1 (Clone EP733Y)
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 481.
Clone NameEP733Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:20,000; IHC: 1:250~500; ICC: 1: 100 -250; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens adducin 1 (alpha) (ADD1), transcript variant 2
Alternative NameADDA
Database LinkNP_054908
FunctionAdducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; a and ß, or a and ? subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - alpha Adducin antibody [EP733Y]; All lanes : Anti-alpha Adducin (phospho S481) antibody [EP733Y] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa lysate+TPA.Lysates/proteins at 10 ug per lane.Predicted band size : 130 kDa.Observed band size : 130 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP733Y]; Immunohistochemical analysis of paraffin-embedded human uterus using anti-alpha Adducin TA300679 at a 1:250 dilution

 

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