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Home Antibody All anti-AFP antibodies

Anti-AFP Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300675
  • Rabbit Polyclonal Antibody against AFP
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400455) , 20ug
100ul 379 3-7 Days
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WB(1)
IHC(1)
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Also for AFP (NM_001134)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human AFP was used as an immunogen.
Clone Name IsotypeIgG
Species ReactivityHuman, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Is unsuitable for Flow Cyt,ICC/IF or IP.

Reference Data

Target NameHomo sapiens alpha-fetoprotein (AFP)
Alternative NameFETA; HPAFP
Database LinkNP_001125
FunctionAlpha-fetoprotein (AFP) is a serum glycoprotein protein produced in the liver or yolk sac of fetal staged mammals. AFP synthesis is minimal after birth and trace amount is expressed in the adult liver (1). AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways (2). AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High level of AFP is also seen some diseases, such as hepatitis and colitis. AFP is used as a screening marker for fetal abnormalities in pregnant women, such as Down syndrome. Recently, AFP has been introduced as an anti-cancer drug-ligand carrier, transporting drugs to target tumor cells, increasing anti-tumor efficiency (3).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - alpha 1 Fetoprotein antibody; Anti-alpha 1 Fetoprotein antibody at 1/50000 dilution + HepG2 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled, at 1/2000 dilution.Predicted band size : 69 kDa.Observed band size : 69 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody; TA300675 staining alpha 1 Fetoprotein in Mouse embryonic liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in 1% BSA in TBS) for 1 hour at 23°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.

 

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