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Anti-GYS1 Antibody EP816Y
Also for GYS1 (NM_002103)
|A synthetic peptide corresponding to residues near the N-terminus of the human glycogen synthase was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:20,000; IHC: 1:250 500; ICC: 1: 100 250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1|
|Glycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-a-D-glucosyl)n to yield UDP and (1,4-a-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.|
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Western blot - Glycogen synthase 1 antibody [EP816Y]; Anti-Glycogen synthase 1 antibody [EP816Y] at 1/20000 dilution + HeLa cell lysate at 10 µg.Predicted band size : 81 kDa.Observed band size : 80 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Glycogen synthase 1 antibody [EP816Y]; Immunohistochemical staining of paraffin-embedded human muscle using anti-Glycogen Synthase 1 ab40867 diluted 1:250.
Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1 antibody [EP816Y]; ICC/IF image of ab40867 stained A431 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.