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Anti-SMAD2 Antibody EP784Y
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Also for SMAD2 (NM_005901)
|A synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as an immunogen.|
||WB: 1:500,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:30; IP: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1|
|hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2|
|Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5). |
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot analysis on Jurkat cell lysate using anti-Smad2, dilution 1:500,000.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Smad2.