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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody EP784Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300654 Rabbit Monoclonal Antibody against SMAD2 (Clone EP784Y) 100ul $325 In Stock
LC401783 SMAD2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as an immunogen.
Clone NameEP784Y IsotypeIgG
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsICC: 1:100 - 1:250; IHC-P: Use at an assay dependent concentration; IP: 1:50; WB: 1:10000; FC: 1:100
Predicted MW Explanation 58 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens SMAD family member 2 (SMAD2), transcript variant 1
Alternative NamehMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database LinkNP_005892
Entrez Gene 4087 Human
Entrez Gene 29357 Rat
FunctionSmad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smad2 antibody [EP784Y]; Anti-Smad2 antibody [EP784Y] at 1/500000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP784Y]; TA300654 at a 1:100 dilution staining Smad2 in human prostate carcinoma tissue.

FC Image
Flow Cytometry - Anti-Smad2 antibody; Overlay histogram showing PC3 cells stained with TA300654 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.


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