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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody EP784Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300654
  • Rabbit Monoclonal Antibody against SMAD2 (Clone EP784Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401783) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for SMAD2 (NM_005901)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as an immunogen.
Clone NameEP784Y IsotypeIgG
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsICC: 1:100 - 1:250; IHC-P: Use at an assay dependent concentration; IP: 1:50; WB: 1:10000; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens SMAD family member 2 (SMAD2), transcript variant 1
Alternative NamehMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database LinkNP_005892
FunctionSmad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Related Pathway
Adherens junction
Cell cycle
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smad2 antibody [EP784Y]; Anti-Smad2 antibody [EP784Y] at 1/500000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP784Y]; TA300654 at a 1:100 dilution staining Smad2 in human prostate carcinoma tissue.
FC Image
Flow Cytometry - Anti-Smad2 antibody [EP784Y]; Overlay histogram showing PC3 cells stained with TA300654 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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