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Anti-SMAD2 Antibody EP784Y

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

WB(1)

IHC(1)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as an immunogen.
Clone Name	EP784Y	Isotype	IgG
Species Reactivity	Human, Mouse	Concentration
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB: 1:500,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:30; IP: 1:50
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1
Alternative Name	hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database Link	NP_005892
Function	Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Related Pathway	Adherens junction Cell cycle EGFR1 Signaling Pathway TGF Beta Signaling Pathway Wnt Signaling Pathway

Related Lysate from OriGene • Transient overexpression lysate of SMAD family member 2 (SMAD2), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • SMAD2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • SMAD2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Smad2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • SMAD2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Smad2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human SMAD family member 2 (SMAD2), transcript variant 1 , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human SMAD family member 2 (SMAD2), transcript variant 1, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1 as 200 ug transfection-ready DNA Related cDNA Clones • Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1 as transfection-ready DNA

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

• HRP
• Biotin
• PE
• FITC
• DyLight® 488
• DyLight® 550
• DyLight® 594
• DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)