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Anti-SMAD2 Antibody EP784Y
Also for SMAD2 (NM_005901)
|A synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent concentration; IP: 1:50; WB: 1:10000; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse
|Homo sapiens SMAD family member 2 (SMAD2), transcript variant 1|
|hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2|
|Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5). |
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Smad2 antibody [EP784Y]; Anti-Smad2 antibody [EP784Y] at 1/500000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP784Y]; ab40855 at a 1:100 dilution staining Smad2 in human prostate carcinoma tissue.
Flow Cytometry - Anti-Smad2 antibody [EP784Y]; Overlay histogram showing PC3 cells stained with ab40855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.