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Home Antibody All anti-HSPD1 antibodies

Anti-HSPD1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300644
  • Rabbit Polyclonal Antibody against HSPD1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400783) , 20ug Explanation
100ul 379 In Stock
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WB(1)
IHC(2)
IF(1)
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Also for HSPD1 (NM_002156)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Heat Shock Protein 60 was used as immunogen.
Clone Name IsotypeIgG
Species ReactivityMouse, Rat, Hamster, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:20000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:20; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens heat shock 60kDa protein 1 (chaperonin) (HSPD1), transcript variant 1
Alternative NameCPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Database LinkNP_002147
Entrez Gene 3329 Human
Entrez Gene 15510 Mouse
Entrez Gene 63868 Rat
FunctionThe 60 kDa heat shock protein (HSP60) is a highly conserved protein that act as a chaperone involved in correct folding of newly synthesized proteins (1-2). Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. In normal cardiac myocytes, cytosolic HSP60 complexes with Bax, Bak and Bcl-XL (3). While over expression of HSP60 prevents apoptosis in cardiac cells; is has also been shown that HSP60 accelerated the activation of Caspase 3 during apoptosis (4-5). Therefore, the role of HSP60 in apoptosis may be both pro- and anti-apoptotic, depending on the cell type, or apoptotic stimulus.
Related PathwayStem cell - PluripotencyDruggable Genome RNA degradationType I diabetes mellitus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Hsp60 antibody; Anti-Hsp60 antibody at 1/50000 dilution + T47D cell lysate at 10 ug.Secondary.Goat anti -rabbit HRP at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 60 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Hsp60 antibody; Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using TA300644 at 1/250 dilution
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp60 antibody; TA300644 staining Hsp60 from human colon tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 21°C. Alexa fluor? 594 goat polyclonal, diluted 1/5000, was used as the secondary.
IF Image
Immunocytochemistry/ Immunofluorescence-Hsp60 antibody(TA300644); ICC/IF image of TA300644 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43?M.

 

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