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Anti-MAPK7 Antibody EP791Y
Also for MAPK7 (NM_139032)
|A synthetic peptide corresponding to residues in the C-terminus of humanERK5 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; ICC: 1:250 - 1:500; FC: 1:100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Is unsuitable for IHC.
|Homo sapiens mitogen-activated protein kinase 7 (MAPK7), transcript variant 2|
|BMK1; ERK4; ERK5; PRKM7|
|The extracellular signal-regulated kinase 5 (ERK5), also known as MAPK7 or big mitogen-activated protein kinase 1 (BMK1), is a member of the MAP kinase subfamily (1). ERK5 differs considerably from other MAPKs; it contains an unusually long carboxyl-terminal tail which might contribute to the regulation of its activity and/or localization. In response to extracellular signals, ERK5 translocates to the nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors (2). ERK5 also differs from other MAPKs in possessing a potent transcriptional activation domain which mediates protein-protein interactions with the myocyte enhancer factor 2 (MEF2) transcription factors (3). ERK5 is specifically activated by MAPK kinase 5 (MAP2K5/MEK5) and plays an important role in mammary epithelial proliferation, endothelial cell survival and normal embryonic development (4-5).|
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - ERK5 antibody [EP791Y]; Anti-ERK5 antibody [EP791Y] at 1/5000 dilution + Hela cell lysate at 10 µg.Predicted band size : 89 kDa.Observed band size : 115 kDa .
Flow Cytometry - Anti-ERK5 antibody [EP791Y]; Overlay histogram showing A549 cells stained with ab40809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.