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Home Antibody All anti-EEF2K antibodies

Anti-EEF2K Antibody EP724Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300638
  • Rabbit Monoclonal Antibody against EEF2K (Clone EP724Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402241) , 20ug
100ul 325 In Stock
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WB(1)
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Also for EEF2K (NM_013302)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the Calmodulin binding region of human eEF2 was used as immunogen.
Clone NameEP724Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:50000; IP: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IHC-P.

Reference Data

Target NameHomo sapiens eukaryotic elongation factor-2 kinase (EEF2K)
Alternative NameeEF-2K; HSU93850
Database LinkNP_037434
FunctionEukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EEF2K antibody [EP724Y]; All lanes : Anti-EEF2K antibody [EP724Y] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 95 kDa .

 

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