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Anti-PPP1R1B Antibody EP721Y
Also for PPP1R1B (NM_032192)
|A synthetic peptide corresponding to residues near the N-term of human DARPP-32 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5,000-20,0000; IHC: 1:250-500; ICC: 1:50-100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (PPP1R1B), transcript variant 1|
|The 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).|
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Western blot - DARPP32 antibody [EP721Y]; Anti-DARPP32 antibody [EP721Y] at 1/20000 dilution + Human brain lysate at 10 µg.Predicted band size : 32 kDa.Observed band size : 32 kDa.
Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody [EP721Y]; Immunohistochemical analysis human gastric adenocarcinoma using ab40802.