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Anti-PPP1R1B Antibody EP720Y
Also for PPP1R1B (NM_032192)
|A synthetic peptide corresponding to residues near the N-term of human DARPP-32 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||IHC-Fr: 1:7500; ICC/IF: Use at an assay dependent concentration; WB: 1:5000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC: 1:50 - 1:100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (PPP1R1B), transcript variant 1|
|The 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).|
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Western blot - DARPP32 antibody [EP720Y]; Anti-DARPP32 antibody [EP720Y] at 1/50000 dilution + Rat brain lysate at 10 µg.Predicted band size : 32 kDa.Observed band size : 32 kDa.
Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody [EP720Y]; Immunohistochemical analysis of human breast adenocarcinoma using ab40801.
Immunohistochemistry (Frozen sections) - DARPP32 antibody [EP720Y]; ab40801 staining DARPP32 in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody (1/7500 in TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to rabbit IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.
IHC - Wholemount - Anti-DARPP32 antibody [EP720Y]; Immunohistochemical analysis of murine brain tissue, staining DARPP32 (red) with ab40801. Slices were fixed in paraformaldehyde and blocked with 5% horse serum and 0.25% Triton X-100. Samples were incubated with primary antibody (1/200) for 15 hours.