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Home Antibody All anti-ADD1 antibodies

Anti-ADD1 Antibody EP734Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300626
  • Rabbit Monoclonal Antibody against ADD1 (Clone EP734Y)
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.
Clone NameEP734Y IsotypeIgG
Species ReactivityHuman, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:5,000-50,000; IHC: 1:250-500; ICC: 1:250-500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens adducin 1 (alpha) (ADD1), transcript variant 2
Alternative NameADDA
Database LinkNP_054908
FunctionAdducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; a and ß, or a and ? subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).
Related Pathway

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WB Image
Western blot - alpha Adducin antibody [EP734Y]; Anti-alpha Adducin antibody [EP734Y] at 1/50000 dilution + HeLa cell lysate at 10 µg.Predicted band size : 120 kDa.Observed band size : 120 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP734Y]; TA300626 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - alpha Adducin antibody [EP734Y]; TA300626, (1/250) staining HeLa cells by immunofluorescence.

 

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