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Anti-ADD1 Antibody EP734Y
Also for ADD1 (NM_014189)
|A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:5,000-50,000; IHC: 1:250-500; ICC: 1:250-500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens adducin 1 (alpha) (ADD1), transcript variant 2|
|Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; a and ß, or a and ? subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).|
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Western blot - alpha Adducin antibody [EP734Y]; Anti-alpha Adducin antibody [EP734Y] at 1/50000 dilution + HeLa cell lysate at 10 µg.Predicted band size : 120 kDa.Observed band size : 120 kDa.
Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP734Y]; ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - alpha Adducin antibody [EP734Y]; ab40760, (1/250) staining HeLa cells by immunofluorescence.