Home All anti-PIK3R1 antibodies
Anti-PIK3R1 Antibody EP380Y
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Also for PIK3R1 (NM_181523)
|A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alpha was used as an immunogen.|
|WB, IHC, IF
||WB: 1:500-2000; IHC: 1: 100-250; ICC: 1:250-500; FC: 1:60; IP: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1|
|GRB1; p85; p85-ALPHA|
|PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; the regulatory subunit p85 and the catalytic subunit p110 (1-2). In quiescent cells p85 maintains p110 in a low activity state. Upon growth factor stimulation, p85 directly interacts with phosphotyrosine residues of activated growth factor receptors or adaptor proteins leading to activation of p110. p85 exists in two isoforms: p85 , including two short forms termed p50 and p55/AS53 and p85 including short form p55 PIK/p55 (3). The p85 adaptor protein uses the inter-SH2 (iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosine residues, and proline-rich motifs to interact with activated tyrosine kinases such as BCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2 (4-5). |
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
Signaling by GPCR
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
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Western blot analysis of anti- PI3-K p85 subunit alpha, dilution 1:2,000. A: A431 B: MCF-7 C: Raji
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PI3-K p85 subunit alpha.
Immunofluorescent staining of HeLa cells using anti- PI3-K p85 subunit alpha.