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Home All anti-PIK3R1 antibodies
Anti-PIK3R1 Antibody EP380Y
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for PIK3R1 (NM_181523)
OriGene Data
| A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alpha was used as an immunogen. |
| EP380Y |
IgG |
| Human, Rat |
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| WB, IHC, IF |
WB: 1:500-2000; IHC: 1: 100-250; ICC: 1:250-500; FC: 1:60; IP: 1:50 |
| 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1 |
| GRB1; p85; p85-ALPHA |
| NP_852664 |
| PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; the regulatory subunit p85 and the catalytic subunit p110 (1-2). In quiescent cells p85 maintains p110 in a low activity state. Upon growth factor stimulation, p85 directly interacts with phosphotyrosine residues of activated growth factor receptors or adaptor proteins leading to activation of p110. p85 exists in two isoforms: p85 , including two short forms termed p50 and p55/AS53 and p85 including short form p55 PIK/p55 (3). The p85 adaptor protein uses the inter-SH2 (iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosine residues, and proline-rich motifs to interact with activated tyrosine kinases such as BCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2 (4-5). |
Apoptosis Delta-Notch Signaling Pathway EGFR1 Signaling Pathway Focal Adhesion Hemostasis Jak-STAT signaling pathway Signaling by GPCR TGF Beta Signaling Pathway Toll-like receptor signaling pathway |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
Western blot analysis of anti- PI3-K p85 subunit alpha, dilution 1:2,000. A: A431 B: MCF-7 C: Raji
IHC Image
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PI3-K p85 subunit alpha.
IF Image
Immunofluorescent staining of HeLa cells using anti- PI3-K p85 subunit alpha.
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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