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Anti-GFAP Antibody EP672Y
Also for GFAP (NM_002055)
|A synthetic peptide corresponding to residues in the C-term of human GFAP was used as immunogen.|
|Rat, Human (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC
||IHC-Fr: 1:250 - 1:1000; WB: 1:2000; IHC-P: Use at an assay dependent concentration; ICC: 1:250 - 1:500
|Does not react with Mouse. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1|
Entrez Gene 2670 Human
Entrez Gene 24387 Rat
|Glial fibrillary acidic protein (GFAP) belongs to the class III of the intermediate filament (IF) proteins highly specific to astrocytes which function as nerve cells in the brain and in the central nervous system (CNS) (1-2). Two other class III IF proteins, nestin and vimentin, dominate in immature astrocytes, while GFAP is most abundant in mature astrocytes (3). GFAP is used as a marker to distinguish astrocytes form other glial cells during development. Mutations in GFAP cause Alexander disease, a rare disorder of the CNS. Most frequent mutations are located at Arginine 79 or 239 (4). |
|ES Cell Differentiation/IPS |
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Western blot - GFAP antibody [EP672Y]; Anti-GFAP antibody [EP672Y] at 1/2000 dilution + Rat brain lysate.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Other-Anti-GFAP antibody [EP672Y](TA300587); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Paraffin-embedded sections) - GFAP antibody [EP672Y]; Immjunohistochemical analysis of paraffin-embedded human brain using TA300587 at 1/250.
Immunohistochemistry (Frozen sections) - GFAP antibody [EP672Y]; TA300587 staining GFAP in a xenograft section, human neural stem cells in fetal mouse, by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde and permeabilized with 0.2% Triton X. Samples were then blocked using 3% normal donkey serum for 1.5 hours at 20°C, then incubated with TA300587 at a 1/1000 dilution for 1 hour at 20°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to Alexa Fluor555, used at a 1/500 dilution..TA300587 only recognizes human astrocytes and none of the murine tissue was stained. The staining of the human GFAP fibers was always very strong, clear and reliable. Very good antibody for xenograft experiments in neuroscience!
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GFAP antibody [EP672Y](TA300587); TA300587 showing positive staining in Normal brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GFAP antibody [EP672Y](TA300587); TA300587 showing positive staining in Astrocytoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GFAP antibody [EP672Y](TA300587); TA300587 showing negative staining in Meningioma tissue.