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Home Antibody All anti-BBC3 antibodies

Anti-BBC3 Antibody EP512Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300574
  • Rabbit Monoclonal Antibody against BBC3 (Clone EP512Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402330) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
FC(1)
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Also for BBC3 (NM_014417)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recog
Clone NameEP512Y IsotypeIgG fraction
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:30 - 1:100; WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens BCL2 binding component 3 (BBC3), transcript variant 4
Alternative NameJFY-1; JFY1; PUMA
Database LinkNP_055232
Entrez Gene 27113 Human
FunctionPUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta.
Related PathwayDruggable Genome p53 signaling pathwayHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PUMA antibody [EP512Y]; Anti-PUMA antibody [EP512Y] at 1/5000 dilution + K562 cell lysate.Predicted band size : 21 kDa.Observed band size : 18 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PUMA antibody [EP512Y]; TA300574 at a 1:100 dilution staining PUMA in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PUMA antibody [EP512Y]; TA300574 at a 1:100 dilution staining PUMA in human ovary carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - PUMA antibody [EP512Y]; TA300574 at a 1:250 dilution staining PUMA in Hela cells using Immunocytochemistry/Immunofluorescence.
FC Image
Flow Cytometry - Anti-PUMA antibody [EP512Y]; Overlay histogram showing K562 cells stained with TA300574 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in K562 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

 

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