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Home All anti-BBC3 antibodies
Anti-BBC3 Antibody EP512Y
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for BBC3 (NM_014417)
OriGene Data
| A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recog |
| EP512Y |
IgG |
| Human |
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| WB, IHC, IF |
WB: 1: 1,000-5,000; IHC: 1: 100-250; ICC: 1:250-500; FC: 1:30; IP: 1:50 |
| 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens BCL2 binding component 3 (BBC3), nuclear gene encoding mitochondrial protein, transcript variant 4 |
| JFY-1; JFY1; PUMA |
| NP_055232 |
| PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta. |
Apoptosis |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
Western blot analysis on K562 cell lysate using anti-PUMA (C-term), dilution 1:5,000.
IHC Image
(A) Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term); (B) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PUMA (C-term)
IF Image
Immunofluorescent staining of HeLa cells using anti-PUMA (C-term).
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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