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Anti-BBC3 Antibody EP512Y
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Also for BBC3 (NM_014417)
|A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recog|
|WB, IHC, IF
||WB: 1: 1,000-5,000; IHC: 1: 100-250; ICC: 1:250-500; FC: 1:30; IP: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens BCL2 binding component 3 (BBC3), nuclear gene encoding mitochondrial protein, transcript variant 4|
|JFY-1; JFY1; PUMA|
|PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta. |
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Western blot analysis on K562 cell lysate using anti-PUMA (C-term), dilution 1:5,000.
(A) Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term); (B) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PUMA (C-term)
Immunofluorescent staining of HeLa cells using anti-PUMA (C-term).