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Anti-TP73 Antibody EP436Y
Also for TP73 (NM_005427)
|A synthetic peptide corresponding to residues near the transactivation domain of human p73 was used as immunogen. p73 has a predicted molecular weight of 73 kDa but is detected at 63kDa.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||IHC-Fr: Use at an assay dependent concentration; WB: 1:500 - 1:2000; ICC/IF: 1:150; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens tumor protein p73 (TP73), transcript variant 1|
|p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over- expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (a, ß, and ?) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5). |
TGF Beta Signaling Pathway
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Western blot - p73 antibody [EP436Y]; All lanes : Anti-p73 [EP436Y] antibody at 1/2000 dilution.Lane 1 : Hela lysate.Lane 2 : Jurkat lysate.Lane 3 : NIH 3T3 lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 70 kDa.Observed band size : 63 kDa .
Immunohistochemistry (Paraffin-embedded sections) - p73 antibody [EP436Y]; Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using 1/100 ab40658
Immunohistochemistry (Frozen sections) - p73 antibody [EP436Y]; ab40658 staining p73 in murine hairy skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with paraformaldehyde, permeabilized using 0.1% Triton then blocked using 5% BSA for 30 minutes at 25°C. Samples were then incubated with ab40658 at a 1/100 dilution for 16 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.