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Anti-EIF4E Antibody Y449
Also for EIF4E (NM_001968)
|A synthetic peptide corresponding to residues in the C-term of human eIF-4E, was used as immunogen. The antibody detects a band on western blot of approximately 28 kDa.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000 - 1:20000; IHC-P: Use at an assay dependent dilution; FC: 1:50; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC.
|Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1|
|AUTS19; CBP; EIF4E1; EIF4EL1; EIF4F|
|eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5). |
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Western blot - eIF4E antibody [Y449]; Anti-eIF4E antibody [Y449] at 1/20000 dilution + 293 cell lysate.Predicted band size : 25 kDa.Observed band size : 30 kDa .
Immunohistochemistry (Paraffin-embedded sections) - eIF4E antibody [Y449]; Ab33768, at 1/250 dilution, staining eIF4E in paraffin embedded human breast carcinoma tissue sections by Immunohistochemistry.
Flow Cytometry - Anti-eIF4E antibody [Y449]; Overlay histogram showing HEK293 cells stained with ab33768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.