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Home Antibody All anti-RPS6KA5 antibodies

Anti-RPS6KA5 Antibody E375

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300536
  • Rabbit Monoclonal Antibody against RPS6KA5 (Clone E375)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403633) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for RPS6KA5 (NM_182398)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 376.
Clone NameE375 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; IP: 1:30; IHC-P: Use at an assay dependent dilution
BufferDoes not react with Mouse. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2
Alternative NameMSK1; MSPK1; RLPK
Database LinkNP_872198
FunctionMSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).
Related Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway

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WB Image
Western blot - MSK1 (phospho S376) antibody [E375]; All lanes : Anti-MSK1 (phospho S376) antibody [E375] at 1/5000 dilution.Lane 1 : Untreated K562 cell lysate.Lane 2 : K562 cell lysate treated with Alkaline.Predicted band size : 90 kDa.Observed band size : 87 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375]; Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

 

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