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Anti-RPS6KA5 Antibody E375
Also for RPS6KA5 (NM_182398)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 376.|
|Rat, Human (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; IP: 1:30; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2|
|MSK1; MSPK1; RLPK|
Entrez Gene 9252 Human
Entrez Gene 314384 Rat
|MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4). |
|Protein KinaseTranscription FactorsDruggable Genome MAPK signaling pathwayNeurotrophin signaling pathwayBladder cancer|
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Western blot - MSK1 (phospho S376) antibody [E375]; All lanes : Anti-MSK1 (phospho S376) antibody [E375] at 1/5000 dilution.Lane 1 : Untreated K562 cell lysate.Lane 2 : K562 cell lysate treated with Alkaline.Predicted band size : 90 kDa.Observed band size : 87 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375]; Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.