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Anti-PRKAB1 Antibody Y367
Also for PRKAB1 (NM_006253)
|A synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent dilution; FC: 1:50; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)|
|The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1). AMPK is a heterotrimeric protein comprising a (63 kDa), ß (38 kDa) and ? (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). |
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Western blot - AMPK beta 1 antibody [Y367]; All lanes : Anti-AMPK beta 1 antibody [Y367] at 1/5000 dilution.Lane 1 : (A) : NIH 3T3.Lane 2 : (B) : Hela.Lane 3 : (C) : A431.Lane 4 : (D) : PC-12.Predicted band size : 30 kDa.Observed band size : 38 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK beta 1 antibody [Y367]; ab32112 at a 1:100 dilution staining AMPK beta 1 in human lung carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry-Anti-AMPK beta 1 antibody [Y367](ab32112); Overlay histogram showing HeLa cells stained with ab32112 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.