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Anti-PRKAG1 Antibody Y308
Also for PRKAG1 (NM_002733)
|A synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit was used as an immunogen|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:20; IP: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC.
|Homo sapiens protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant 1|
|The 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising a (63 kDa), ß (38 kDa) and ? (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3). |
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Western blot - AMPK gamma 1 antibody [Y308]; Anti-AMPK gamma 1 antibody [Y308] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 38 kDa.Observed band size : 38 kDa.
Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308]; AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) . Bands: 37kDa: AMPK gamma 1.