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Anti-APP Antibody Y188
|A synthetic peptide corresponding to residues in the NPXY motif of human Amyloid Beta A4 precusor (APP) was used as immunogen. This antibody specifically recognizes APP and its isoforms. It will not recognize either of the two Amyloid Beta Protein ( Aß4 )|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:100 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC: 1:250 - 1:500; FC: 1:100; IP: 1:50; IHC-FoFr: 1:750; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens amyloid beta (A4) precursor protein (APP), transcript variant 3|
|AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2|
Entrez Gene 351 Human
Entrez Gene 11820 Mouse
Entrez Gene 54226 Rat
|Alzheimer’s disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (Aß4) protein has been found to be the principal constituent of senile plaques of AD’s patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa Aß4 protein. Two variants of Aß4 can be detected; Aß1-40 “short-tailed” and Aß1-42 “long-tailed” (3-4). |
|TransmembraneDruggable Genome Alzheimer's disease|
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Western blot - Amyloid beta precursor protein antibody [Y188]; Anti-Amyloid beta precursor protein antibody [Y188] at 1/20000 dilution + Hela cell lysate.Predicted band size : 87 kDa.Observed band size : 95 kDa .Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Amyloid beta precursor protein antibody [Y188]; Anti-Amyloid beta precursor protein antibody [Y188] at 1/1000 dilution + Mouse spleen whole cell lysate at 100 ug.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 87 kDa.Observed band size : 95 kDa .Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.Exposure time : 10 minutes
Immunohistochemistry (Paraffin-embedded sections) - Amyloid beta precursor protein antibody [Y188]; Ab32136, at a 1/250 dilution, staining Amyloid beta precursor protein by immunohistochemistry. .Positive immunohistochemical staining, using paraffin embedded human brain tissue (A). . Negative immunohistochemical staining, using human breast (B), skeletal muscle (C) and liver (D) tissues. .Tissues were stained in parallel on the same Normal Tissue Array.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Amyloid beta precursor protein antibody [Y188]; TA300496, staining Amyloid beta precursor protein (green) in spinal cords from wild-type (left) or APP-/- (right) mouse embryos by Immunohistochemistry (PFA perfusion fixed frozen sections). . Embryos were fixed overnight in 4% paraformaldehyde at 4Â°C, permeabilized for 30 min in PBS containing 0.1% Triton X-100 and blocked for 4 h in PBS containing 3% normal donkey serum, 2% BSA, and 0.1% Triton X-100. Sections were incubated overnight at 4 Â°C with anti-APP antibody at 1/200 dilution. An AlexaFluor?488-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody.
Flow Cytometry - Anti-Amyloid beta precursor protein antibody [Y188]; Overlay histogram showing PC12 cells stained with TA300496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in PC12 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.