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Anti-HSPB1 Antibody Y175
Also for HSPB1 (NM_001540)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27 was used as immunogen. The antibody will detect Src phosphorylation on Serine 78.|
||0.5~1.0 mg/ml (Lot Dependent)
|IHC, IF, DB
||WB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; IP: 1:50; IHC-Fr: Use at an assay dependent dilution
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt.
|Homo sapiens heat shock 27kDa protein 1 (HSPB1)|
|CMT2F; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27|
|Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4). |
MAPK signaling pathway
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Immunohistochemistry (Paraffin-embedded sections) - Hsp27 (phospho S78) antibody [Y175]; Immunohistochemical analysis of Hsp27 phospho S78 expression in paraffin embedded human breast carcinoma using 1/250 TA300477.
Immunocytochemistry/ Immunofluorescence - Hsp27 (phospho S78) antibody [Y175]; Immunofluorescent analysis of Hsp27 phospho S78 expression in HeLa cells using 1/250 TA300477.
Dot Blot - Hsp27 (phospho S78) antibody [Y175]; Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using TA300477 at dilution 1/2000.