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Home All anti-HSPB1 antibodies
Anti-HSPB1 Antibody Y175
Also for HSPB1 (NM_001540)
Homo sapiens heat shock 27kDa protein 1 (HSPB1)
CMT2F; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
A synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27 was used as immunogen. The antibody will detect Src phosphorylation on Serine 78.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
WB, IHC, IF, DB
WB: 1:2,000; IHC: 1:250-500; ICC: 1:250-500; IP: 1:50
Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
MAPK signaling pathway
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Western blot analysis on A431 cell lysates using anti- Phospho-HSP27 (pS78), 1:2,000 dilution. Cells were either (A) untreated (B) treated with Anisomycin
Immunohistochemical analysis of paraffin embedded human bhreast carcinoma using anti-Phospho-HSP27 (pS78)
Immunofluorescent staining of HeLa cells using anti-Phospho-HSP27 (pS78).
Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti- Phospho-HSP27 (pS78) dilution 1:2,000