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Home Antibody All anti-HSPB1 antibodies

Anti-HSPB1 Antibody Y175

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300477
  • Rabbit Monoclonal Antibody against HSPB1 (Clone Y175)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400587) , 20ug Explanation
100ul 325 3-7 Days
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IHC(1)
IF(1)
DB(1)
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Also for HSPB1 (NM_001540)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27 was used as immunogen. The antibody will detect Src phosphorylation on Serine 78.
Clone NameY175 IsotypeIgG fraction
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *IHC, IF, DB Suggested DilutionsWB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; IP: 1:50; IHC-Fr: Use at an assay dependent dilution
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens heat shock 27kDa protein 1 (HSPB1)
Alternative NameCMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
Database LinkNP_001531
Entrez Gene 3315 Human
FunctionHeat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
Related Pathway MAPK signaling pathwayVEGF signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Hsp27 (phospho S78) antibody [Y175]; Immunohistochemical analysis of Hsp27 phospho S78 expression in paraffin embedded human breast carcinoma using 1/250 TA300477.
IF Image
Immunocytochemistry/ Immunofluorescence - Hsp27 (phospho S78) antibody [Y175]; Immunofluorescent analysis of Hsp27 phospho S78 expression in HeLa cells using 1/250 TA300477.
DB Image
Dot Blot - Hsp27 (phospho S78) antibody [Y175]; Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using TA300477 at dilution 1/2000.

 

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