Homo sapiens tumor protein p53 (TP53), transcript variant 1
Synonyms:
BCC7; LFS1; P53; TRP53
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Serine 6 of human p53 was used as immunogen. The antibody will detect p53 phosphorylation on Serine 6. Predicted to react with p53 beta and gamma isoform, based on sequence homology.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5).
Related Pathway
Apoptosis
Cell cycle
Delta-Notch Signaling Pathway
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot analysis on MCF7 cell lysates using anti-Phospho-p53 (pS6), 1:5,000 dilution. Cells were either (A) non treated or treated with Actinomycin D for (B) 3 hrs, (C) 6 hrs, and (D) 18hrs
