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Anti-TERT Antibody Y182

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SKU Description Amount Price Availability*  
TA300468
  • Rabbit Monoclonal Antibody against TERT (Clone Y182)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403673) , 20ug
100ul 325 In Stock
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WB(1)
IF(2)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of human TERT was used as immunogen.
Clone NameY182 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000; ICC/IF: 1:50 - 1:100; FC: 1:100; IP: Use at an assay dependent dilution; IHC-P: Use at an assay dependent dilution; IHC-Fr: Use a concentration of 0.75 ug/ml
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens telomerase reverse transcriptase (TERT), transcript variant 1
Alternative NameCMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TP2; TRT
Database LinkNP_937983
FunctionHuman telomerase reverse transcriptase (hTERT) is the catalytic subunit of the telomerase (1-2). It is also responsible for addition of TTAGGG repeats to the telomere ends of the chromosome, which is important in maintaining cell immortality. (3). In most normal human cells the expression of hTERT is very low. On the other hand, greater than 90 % of human tumors of different pathology and origins have been shown to upregulate telomerase expression (4), making hTERT a cancer-associated target
Related Pathway

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WB Image
Western blot - Telomerase antibody [Y182]; Anti-Telomerase reverse transcriptase antibody [Y182] at 1/1000 dilution + Hela cell lysate.Predicted band size : 127 kDa.Observed band size : 122 kDa .
IF Image
Immunofluorescence - Telomerase antibody [Y182]; Ab32020, at a 1/50 dilution, staining Telomerase Reverse Transcriptase in HeLa cells by Immunofluorescence.
IF Image
Immunocytochemistry/ Immunofluorescence - Telomerase antibody [Y182]; TA300468 stainingTelomerase inhuman breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5%Triton x100and blocking with 2% serumwas performed for 30 minutes in 370C. Samples were incubated with primary antibody (1/100) in PBS for 1 hour30 minutes at 37°C. An Alexa Fluor? 594-conjugatedgoat monoclonal torabbit IgG was used at dilution at1/75 as secondary antibody.

 

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