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Anti-PRKAR2A Antibody Y116
Also for PRKAR2A (NM_004157)
|A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit was used as immunogen. The antibody does not cross-react with other cAMP-dependent kinase.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500~1000; IHC: 1: 100-250; ICC: 1: 100-250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A)|
|PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3). |
TGF Beta Signaling Pathway
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Western blot - PKA R2 antibody [Y116]; Anti-PKA R2 antibody [Y116] at 1/50000 dilution + K562 cell lysate .Predicted band size : 45 kDa.Observed band size : 52 kDa .
Immunohistochemistry (Paraffin-embedded sections) - PKA R2 antibody [Y116]; Immunohistochemical analysis of paraffin-embedded human normal breast tissue using ab32514 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - PKA R2 antibody [Y116]; Immunofluorescent staining of HeLa cells using ab32514 at 1/100 dilution.