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Anti-GRB2 Antibody Y237
Also for GRB2 (NM_002086)
|A synthetic peptide corresponding to residues in the C-terminus of human GRB2 was used as an immunogen. This antibody can also detect the splice isoform GRB3-3 of GRB2.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:5,000-10,000; IHC: 1: 100-250; ICC: 1:50-100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Homo sapiens growth factor receptor-bound protein 2 (GRB2), transcript variant 1|
|ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2|
|The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3). |
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
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Western blot - GRB2 antibody [Y237]; Anti-GRB2 antibody [Y237] at 1/10000 dilution + 293 cell lysate.Predicted band size : 25 kDa.Observed band size : 25 kDa.
Western blot - GRB2 antibody [Y237]; Anti-GRB2 antibody [Y237] at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/2000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 25 kDa.Observed band size : 24 kDa .Exposure time : 1 minute
Western blot - Anti-GRB2 antibody [Y237]; Anti-GRB2 antibody [Y237] at 1/5000 dilution + Active human AHA1 full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 2 minutes
Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y237]; Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 TA300457.
Immunofluorescence - GRB2 antibody [Y237]; Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 TA300457.