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Anti-EIF2AK2 Antibody Y117
Also for EIF2AK2 (NM_002759)
|A synthetic peptide corresponding to residues near C-terminus of human PKR was used as immunogen. The antibody does not cross-react with other GCN2 family members.|
||Protein A purified
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; IP: 1:80
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt.
|Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1|
|EIF2AK1; PKR; PPP1R83; PRKR|
|Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). |
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Western blot - PKR antibody [Y117]; Anti-PKR antibody [Y117] at 1/10000 dilution + MCF-7 cell lysate.Predicted band size : 62 kDa.Observed band size : 68 kDa .
Immunohistochemistry (Paraffin-embedded sections) - PKR antibody [Y117]; Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TA300452 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - PKR antibody [Y117]; Immunofluorescent staining of HeLa cells using TA300452 at 1/250 dilution.