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Anti-NBN Antibody Y112
|A synthetic peptide corresponding to residues near C-terminus of human Nibrin was used as immunogen|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500; IP: 1:60; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:50; FC: 1:1000; ChIP/Chip: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens nibrin (NBN)|
|AT-V1; AT-V2; ATV; NBS; NBS1; P95|
|Nibrin (also known as NBS1 or p95) contains 2 modules found in cell cycle check point; a forkhead-associated domain & a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 & Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair & telomere maintenance in eukaryotic cells(2-4). Mutation in Nibrin & Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) & Ataxia-Telangiectasia- like disorder (ATLD). |
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Western blot - p95 NBS1 antibody [Y112]; Anti-p95 NBS1 antibody [Y112] - ChIP Grade at 1/500 dilution + HeLa cell lysate.Predicted band size : 85 kDa.Observed band size : 100 kDa .
Immunohistochemistry (Paraffin-embedded sections) - p95 NBS1 antibody [Y112]; Immunohistochemical analysis of p95 NBS1 expression in paraffin embedded human skin carcinoma tissue section, using 1/50 ab32074.
Immunocytochemistry/ Immunofluorescence - p95 NBS1 antibody [Y112]; Immunofluorescent analysis of p95 NBS1 in HeLa cell culture, using 1/50 ab32074.
Flow Cytometry - Anti-p95 NBS1 antibody [Y112] - ChIP Grade; Overlay histogram showing HeLa cells stained with ab32074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.