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Anti-PXN Antibody Y113

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SKU Description Amount Price Availability*  
TA300443
  • Rabbit Monoclonal Antibody against PXN (Clone Y113)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401010) , 20ug
100ul 325 3-7 Days
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WB(2)
IHC(4)
IF(5)
Assay(1)
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Also for PXN (NM_002859)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in N-terminus of human Paxillin was used as immunogen. This antibody detects alpha, beta and gamma isoforms of Paxillin.
Clone NameY113 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250; IP: 1:200
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens paxillin (PXN), transcript variant 1
Alternative NameFLJ16691
Database LinkNP_002850
FunctionPaxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion

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WB Image
Western blot - Paxillin antibody [Y113]; Anti-Paxillin antibody [Y113] at 1/10000 dilution + HeLa cell lysate.Predicted band size : 68 kDa.Observed band size : 60 kDa .
WB Image
Western blot - Paxillin antibody [Y113]; Anti-Paxillin antibody [Y113] at 1/5000 dilution + Mouse RAW264.7 whole cell lysate at 20 µg.Secondary.An HRP Donkey anti-rabbit IgG polyclonal at 1/10000 dilution.Predicted band size : 68 kDa.This image is courtesy of an Abreview submitted by Dr. Alban Gaultier
Assay Image
Other-Anti-Paxillin antibody [Y113](TA300443); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Paxillin antibody [Y113]; Immunohistochemical analysis of paxillin expression in paraffin embedded breast carcinoma tissue section, using 1/100 TA300443.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Paxillin antibody [Y113](TA300443); TA300443 showing positive staining in Normal ovary tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Paxillin antibody [Y113](TA300443); TA300443 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [Y113]; TA300443 showing positive staining in Transitional cell carcinoma of kidney tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113]; TA300443 staining paxillin in MEF1 cells treated with (S)-(-)-Blebbistatin , by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (S)-(-)-Blebbistatin , as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ( (S)-(-)-Blebbistatin ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300443 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113]; Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic fibroblastsPreparation:Fix in 3% PFA in PBS for 30 min at RTPrimary antibody: Rabbit anti-paxillin Y113(TA300443), 1:100Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor? 488) pre-adsorbed , 1:200Rhodamine-phalloidin, 1:100Nuclei were counterstained with DAPI
IF Image
Immunocytochemistry/ Immunofluorescence - Paxillin antibody [Y113]; Immunofluorescent analysis of paxillin expression in HeLa cell culture, using 1/250 TA300443.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113]; Immunofluorescence analysis of bovine kidney cells, staining Paxillin with TA300443. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 1 hour. Samples were incubated with primary antibody (1/2500 in 5% BSA) for 1 hour at 25°C. An undiluted AlexaFluor?488-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-Paxillin antibody [Y113](TA300443); TA300443 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin , by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300443 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

 

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