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Home Antibody All anti-PXN antibodies

Anti-PXN Antibody Y113

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SKU Description Amount Price Availability*  
TA300443
  • Rabbit Monoclonal Antibody against PXN (Clone Y113)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401010) , 20ug Explanation
100ul $325 3-7 Days
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WB(2)
IHC(4)
IF(5)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in N-terminus of human Paxillin was used as immunogen. This antibody detects alpha, beta and gamma isoforms of Paxillin.
Clone NameY113 IsotypeIgG
Species ReactivityMouse, Rat, Cow, Dog, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250; IP: 1:200
Predicted MW Explanation 68 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens paxillin (PXN), transcript variant 1
Alternative NameFLJ16691
Database LinkNP_002850
Entrez Gene 5829 Human
Entrez Gene 19303 Mouse
Entrez Gene 360820 Rat
Entrez Gene 486299 Dog
FunctionPaxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
Related PathwayStem cell - PluripotencyDruggable Genome Chemokine signaling pathwayVEGF signaling pathwayFocal adhesionLeukocyte transendothelial migrationRegulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Paxillin antibody [Y113]; Anti-Paxillin antibody [Y113] at 1/10000 dilution + HeLa cell lysate.Predicted band size : 68 kDa.Observed band size : 60 kDa .
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Western blot - Paxillin antibody [Y113]; Anti-Paxillin antibody [Y113] at 1/5000 dilution + Mouse RAW264.7 whole cell lysate at 20 ug.Secondary.An HRP Donkey anti-rabbit IgG polyclonal at 1/10000 dilution.Predicted band size : 68 kDa.
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Other-Anti-Paxillin antibody [Y113](TA300443); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Paraffin-embedded sections) - Paxillin antibody [Y113]; Immunohistochemical analysis of paxillin expression in paraffin embedded breast carcinoma tissue section, using 1/100 TA300443.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Paxillin antibody [Y113](TA300443); TA300443 showing positive staining in Normal ovary tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Paxillin antibody [Y113](TA300443); TA300443 showing positive staining in Ovarian carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [Y113]; TA300443 showing positive staining in Transitional cell carcinoma of kidney tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113]; TA300443 staining paxillin in MEF1 cells treated with (S)-(-)-Blebbistatin , by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (S)-(-)-Blebbistatin , as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ( (S)-(-)-Blebbistatin ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300443 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113]; Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic fibroblastsPreparation:Fix in 3% PFA in PBS for 30 min at RTPrimary antibody: Rabbit anti-paxillin Y113(TA300443), 1:100Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor? 488) pre-adsorbed , 1:200Rhodamine-phalloidin, 1:100Nuclei were counterstained with DAPI
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Immunocytochemistry/ Immunofluorescence - Paxillin antibody [Y113]; Immunofluorescent analysis of paxillin expression in HeLa cell culture, using 1/250 TA300443.
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113]; Immunofluorescence analysis of bovine kidney cells, staining Paxillin with TA300443. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 1 hour. Samples were incubated with primary antibody (1/2500 in 5% BSA) for 1 hour at 25°C. An undiluted AlexaFluor?488-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence-Anti-Paxillin antibody [Y113](TA300443); TA300443 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin , by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300443 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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