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Anti-CREB1 Antibody E306
Also for CREB1 (NM_134442)
|A synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen. This antibody can also recognize the splice isoform of CREB. Predicted to cross-react with bovine, based on sequence homology.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500 - 1:1000; ICC/IF: 1:250 - 1:500; IHC-P: Use at an assay dependent dilution; FC: 1:40; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant B|
|CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3). |
EGFR1 Signaling Pathway
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Western blot - CREB antibody [E306]; Anti-CREB antibody [E306] at 1/1000 dilution + A431 cell lysate.Predicted band size : 37 kDa.Observed band size : 40 kDa .
Immunohistochemistry (Paraffin-embedded sections) - CREB antibody [E306]; Ab32515, at a 1/500 dilution, staining CREB in paraffin embedded prostatic carcinoma tissue sections by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - CREB antibody [E306]; Ab32515, at a 1/500 dilution, staining CREB in PC12 cells by Immunofluorescence.
Flow Cytometry - CREB antibody [E306]; Overlay histogram showing HEK293 cells stained with ab32515 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.