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Anti-CREB1 Antibody E306
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Also for CREB1 (NM_134442)
|A synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen. This antibody can also recognize the splice isoform of CREB. Predicted to cross-react with bovine, based on sequence homology.|
|WB, IHC, IF
||WB: 1:500-1,000; IHC: 1:250-500; ICC: 1:250-500; FC: 1:40; IP: 1:80
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant B|
|CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3). |
EGFR1 Signaling Pathway
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Western blot analysis on A431 cell lysate using anti-CREB, dilution 1:1,000.
Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-CREB.
Immunofluorescent staining of PC-12 cells using anti-CREB.