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Anti-HSP90AB1 Antibody E296
Also for HSP90AB1 (NM_007355)
|A synthetic peptide corresponding to residues in N-terminus of human Hsp90 ß was used as immunogen. Predicted to cross-react with horse, based on sequence homology.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50; FC: 1:10; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), transcript variant 2|
|D6S182; HSP84; HSP90B; HSPC2; HSPCB|
|The 90-kDa heat shock protein (Hsp90) is a highly conserved, and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine and serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform , (Hsp90 a and Hsp90 ß ) can be found in nearly equal amount in humans, and operates as part of a multichaperone machinery in the cytosol, which includes Hsp70, peptidyl-prolyl isomerases and other cochaperones (3).|
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Western blot - Hsp90 beta antibody [E296]; Anti-Hsp90 beta antibody [E296] at 1/500 dilution + Hela cell lysate.Predicted band size : 83 kDa.Observed band size : 92 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Hsp90 beta antibody [E296]; Immunohistochemical analysis of paraffin-embedded urinary bladder carcinoma using ab32568 at 1/50 dilution.