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Home Antibody All anti-CASP3 antibodies

Anti-CASP3 Antibody E83-103


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300422 Rabbit Monoclonal Antibody against CASP3 (Clone E83-103) 100ul $325 In Stock
LC403215 CASP3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases.
Clone NameE83-103 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: Use a concentration of 5 ug/ml; WB: 1:10000; IHC-P: Use at an assay dependent dilution; IP: 1:100; FC: 1:50; ICC: 1:250
Predicted MW Explanation 31 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Does not react with Rat

Reference Data

Target NameHomo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
Alternative NameCPP32; CPP32B; SCA-1
Database LinkNP_116786
Entrez Gene 836 Human
Entrez Gene 12367 Mouse
FunctionCaspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Related PathwayES Cell Differentiation/IPSProteaseDruggable Genome MAPK signaling pathwayp53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseParkinson's diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - pro Caspase 3 antibody [Y83-103]; All lanes : Anti-pro Caspase 3 antibody [E83-103] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin .Predicted band size : 31 kDa.Observed band size : 35 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma TA300422 at 1/250 dilution.

IF Image
ICC/IF image of TA300422 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

FC Image
Flow Cytometry-Anti-pro Caspase 3 antibody(TA300422); Overlay histogram showing Jurkat cells stained with TA300422 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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