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Anti-RPS6KA1 Antibody E239
Also for RPS6KA1 (NM_002953)
|A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human p90RSK1 was used as immunogen. The antibody only detects RSK1 phosphorylated on Serine 380.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1: 10,000; ICC: 1:250-500; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - RSK1 p90 (phospho S380) antibody [E239]; All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] at 1/10000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with EGF .Predicted band size : 83 kDa.Observed band size : 90 kDa .
Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239]; .Predicted band size : 83 kDa.Image from Tangredi MM et al., J Biol Chem. 2012 May 11;287(20):16748-58. Epub 2012 Mar 23.; Fig 4.; doi: 10.1074/jbc.M111.315929; May 11, 2012, The Journal of Biological Chemistry, 287, 16748-16758.
Immunocytochemistry/ Immunofluorescence - RSK1 p90 (phospho S380) antibody [E239]; Immunofluorescent staining of A431 cells using TA300415 at 1/250 dilution.