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Anti-LBR Antibody E398L
|A synthetic peptide corresponding to residues of human Lamin B Receptor was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IP: 1:50; WB: 1:500; ICC/IF: 1:500; IHC-P: Use a concentration of 5 ug/ml
|Does not react with Mouse. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens lamin B receptor (LBR), transcript variant 1|
|DHCR14B; LMN2R; PHA; TDRD18|
|Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4). |
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Western blot - Lamin B Receptor antibody [E398L]; Anti-Lamin B Receptor antibody [E398L] at 1/500 dilution + Jurkat cell lysate.Predicted band size : 71 kDa.Observed band size : 67 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lamin B Receptor antibody [E398L]; IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Lamin B Receptor antibody [E398L]; Ab32535, at a 1/500 dilution, staining Lamin B Receptor in HeLa cells by Immunofluorescence.