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Anti-STAT5A Antibody E289
Also for STAT5A (NM_003152)
|A synthetic peptide corresponding to residues in C-terminus of human Stat5a was used as immunogen. The antibody does not cross-react with other Stat family members.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100; FC: 1:10; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - STAT5a antibody [E289]; Anti-STAT5a antibody [E289] at 1/1000 dilution + A431 cell lysate.Predicted band size : 91 kDa.Observed band size : 92 kDa .
Western blot ; Anti-STAT5a antibody [E289] at 1/1000 dilution + Human STAT5a full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 20 seconds
Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody [E289]; TA300399 at 1/250 dilution, staining human squamous lung carcinoma by Immunohistochemistry, paraffin-embedded tissue.
Flow Cytometry-Anti-STAT5a antibody [E289](TA300399); Overlay histogram showing Jurkat cells stained with TA300399 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.