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Anti-CHEK1 Antibody E250
Also for CHEK1 (NM_001274)
|A synthetic peptide corresponding to N-terminal residues of human Chk1 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1,000; ICC: 1:50; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3|
|Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).|
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Western blot - Chk1 antibody [E250]; Anti-Chk1 antibody [E250] at 1/1000 dilution + K562 cell lysate.Predicted band size : 54 kDa.Observed band size : 54 kDa.