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Home Antibody All anti-MAP2K2 antibodies

Anti-MAP2K2 Antibody Y78

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300386
  • Rabbit Monoclonal Antibody against MAP2K2 (Clone Y78)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403069) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for MAP2K2 (NM_030662)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. Predicted to cross-react with mouse, based on sequence homology.
Clone NameY78 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:250 - 1:500; WB: 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:100; IP: 1:100
Predicted MW Explanation 44 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Does not react with Rat

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)
Alternative NameCFC4; MAPKK2; MEK2; MKK2; PRKMK2
Database LinkNP_109587
Entrez Gene 5605 Human
Entrez Gene 26396 Mouse
FunctionMEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayVascular smooth muscle contractionVEGF signaling pathwayGap junctionToll-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MEK2 antibody [Y78]; Anti-MEK2 antibody [Y78] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 44 kDa.Observed band size : 45 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MEK2 antibody [Y78]; Immunohistochemical analysis of paraffin embedded prostate carcinoma using TA300386 at a dilution of 1/250.
IF Image
Immunocytochemistry/ Immunofluorescence - MEK2 antibody [Y78]; Immunofluorescent staining of HeLa cells using TA300386 at a dilution of 1/250.
FC Image
Flow Cytometry - MEK2 antibody [Y78]; Overlay histogram showing HeLa cells stained with TA300386 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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