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Anti-MAP2K2 Antibody Y78
Also for MAP2K2 (NM_030662)
|A synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. Predicted to cross-react with mouse, based on sequence homology.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:250 - 1:500; WB: 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:100; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Does not react with Rat
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Western blot - MEK2 antibody [Y78]; Anti-MEK2 antibody [Y78] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 44 kDa.Observed band size : 45 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MEK2 antibody [Y78]; Immunohistochemical analysis of paraffin embedded prostate carcinoma using TA300386 at a dilution of 1/250.
Immunocytochemistry/ Immunofluorescence - MEK2 antibody [Y78]; Immunofluorescent staining of HeLa cells using TA300386 at a dilution of 1/250.
Flow Cytometry - MEK2 antibody [Y78]; Overlay histogram showing HeLa cells stained with TA300386 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.