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Home Antibody All anti-ESR1 antibodies

Anti-ESR1 Antibody E115

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300376
  • Rabbit Monoclonal Antibody against ESR1 (Clone E115)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400046) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
Assay(1)
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Also for ESR1 (NM_000125)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding Ser104 and Ser106 of human ERalpha was used as immunogen.
Clone NameE115 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsICC/IF: 1:100; FC: 1:1000; ChIP: Use at an assay dependent concentration; WB: 1:500; IHC-P: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens estrogen receptor 1 (ESR1), transcript variant 1
Alternative NameER; Era; ESR; ESRA; ESTRR; NR3A1
Database LinkNP_000116
FunctionEstrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha (2). Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
Related Pathway
TGF Beta Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Estrogen Receptor alpha antibody [E115]; Anti-Estrogen Receptor alpha [E115] antibody at 1/500 dilution + MCF-7 cell lysate.Predicted band size : 67 kDa.Observed band size : 60 kDa .
Assay Image
ChIP - Anti-Estrogen Receptor alpha antibody [E115]; ChIP analysis using TA300376 binding Estrogen Receptor alpha in MCF7 cells derived from Human breast carcinoma. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4C. Protein binding was detected using real-time PCR. .Positive control: Estrogen treated MCF7 cells tested at PS2 promoter.Negative Control:IgG ChIP and ethanol-depleted cells tested at PS2 promoter
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Receptor alpha antibody [E115]; Immunohistochemical analysis of human breast carcinoma using anti-Estrogen Rreceptor alpha diluted 1:50
FC Image
Flow Cytometry - Anti-Estrogen Receptor alpha antibody [E115]; Overlay histogram showing MCF7 cells stained with TA300376 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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