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Anti-ESR1 Antibody E115
Also for ESR1 (NM_000125)
|A synthetic peptide corresponding to residues surrounding Ser104 and Ser106 of human ERalpha was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||ICC/IF: 1:100; FC: 1:1000; ChIP: Use at an assay dependent concentration; WB: 1:500; IHC-P: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1|
|ER; Era; ESR; ESRA; ESTRR; NR3A1|
|Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha (2). Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).|
TGF Beta Signaling Pathway
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Western blot - Estrogen Receptor alpha antibody [E115]; Anti-Estrogen Receptor alpha [E115] antibody at 1/500 dilution + MCF-7 cell lysate.Predicted band size : 67 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Receptor alpha antibody [E115]; Immunohistochemical analysis of human breast carcinoma using anti-Estrogen Rreceptor alpha diluted 1:50
Flow Cytometry - Anti-Estrogen Receptor alpha antibody [E115]; Overlay histogram showing MCF7 cells stained with ab32063 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ChIP - Anti-Estrogen Receptor alpha antibody [E115]; ChIP analysis using ab32063 binding Estrogen Receptor alpha in MCF7 cells derived from Human breast carcinoma. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4°C. Protein binding was detected using real-time PCR. .Positive control: Estrogen treated MCF7 cells tested at PS2 promoter.Negative Control:IgG ChIP and ethanol-depleted cells tested at PS2 promoter.