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Anti-DAXX Antibody E94
Also for DAXX (NM_001350)
|A synthetic peptide corresponding to residues of human DAXX was used as immunogen. Predicted to cross-react with rat, based on sequence homology.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:50; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens death-domain associated protein (DAXX), transcript variant 2|
|BING2; DAP6; EAP1|
|Daxx is a nuclear protein that has been found to the enhance Fas-mediated apoptosis and activate the JNK pathway (1). Apoptosis signal-regulating kinase 1 (ASK1) controls the dual function of Daxx as a transcriptional repressor in the nucleus and as a proapoptotic signal mediator in the cytoplasm (2). Daxx associates with various proteins, including promyelocytic leukemia protein (PML) and heat shock protein 27, lending significance to their roles in tumor suppression and Daxx-mediated apoptosis (3,4).|
MAPK signaling pathway
TGF Beta Signaling Pathway
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Western blot - Daxx antibody [E94]; Anti-Daxx antibody [E94] at 1/5000 dilution + Hela cell lysate.Predicted band size : 81 kDa.Observed band size : 100 kDa .Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Daxx antibody [E94]; Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-Daxx antibody [E94]; Overlay histogram showing HeLa cells stained with ab32140 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.