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Anti-PXN Antibody E228
|A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:5000; IHC: 1: 100-1:250; ICC: 1:250; IP: 1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens paxillin (PXN), transcript variant 1|
|Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).|
EGFR1 Signaling Pathway
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Western blot - Paxillin (phospho Y31) antibody [E228]; All lanes : Anti-Paxillin (phospho Y31) antibody [E228] at 1/10000 dilution.Lane 1 : untreated NIH 3T3 cell lysate.Lane 2 : PDGF treated NIH 3T3 cell lysate.Predicted band size : 64 kDa.Observed band size : 64 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Paxillin (phospho Y31) antibody [E228]; Immunohistochemical analysis of Paxillin (phospho Y31) expression in paraffin-embedded human colon carcinoma using 1/100 ab32115.
Immunocytochemistry/ Immunofluorescence - Paxillin (phospho Y31) antibody [E228]; Immunofluorescent analysis of Paxillin (phospho Y31) expression in HeLa cell culture, using 1/250 ab32115.
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody [E228]; Immunofluorescence analysis of HeLa cells, staining Paxillin (phospho Y31) with ab32115. Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.