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Anti-MAPT Antibody E178
Also for MAPT (NM_005910)
|A synthetic phospho-peptide corresponding to residues surrounding Ser396 of human Tau was used as immunogen. The antibody only detects Tau phosphorylated on Serine 396. Predicted to cross-react with bovine, based on sequence homology.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||IHC-P: 1:500 - 1:1000; WB: 1:5000; ICC: 1:250; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens microtubule-associated protein tau (MAPT), transcript variant 2|
|DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU|
Entrez Gene 4137 Human
Entrez Gene 17762 Mouse
Entrez Gene 29477 Rat
|Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Taus association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4). |
|Druggable Genome MAPK signaling pathwayAlzheimer's disease|
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Western blot - Anti-Tau antibody [E178]; All lanes : Anti-Tau antibody [E178] at 1/1000 dilution.Lane 1 : Untreated SH-SY5Y cell lysate.Lane 2 : SH-SY5Y cell lysate SH SY5Y cell lysate treated with Oka/CalA. Cells are serum-starved overnight, and then treated with 1nM calyculin A and 500nM Okadaic acid for 2 hours at 37°C.Predicted band size : 79 kDa.
IHC of Tau antibodyon formaldehyde fixed paraffin-embedded rat tongue sections.Antigen retrieval step: Heat mediated.Buffer Used: Citric acid pH6. Permeabilization: None.Primary antibody incubated at1/1000 for2 hours@ 21°C inTBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated toBiotin(1/200). Surrounding these are collagen fibres (C), adipocytes (A) and skeletal/striated muscle fibres in L/S ( M- showing blue myocyte nuclei arranged around the periphery of each fibre).
IHC of Tau antibodyon formaldehyde-fixed paraffin-embedded human salivary gland sections. Antigen retrieval step: Heat mediated.Buffer Used: Citric acid pH6. Permeabilization: No. Blocking step: 1% BSAfor 10 mins at 21°C.TA300315 incubated at 1/1000 for 2 hours at 21°C in TBS/BSA/azide. Secondary antibody: anti rabbit IgG conjugated to Biotin (1/200).
Immunohistochemistry (Paraffin-embedded sections) - Anti-Tau antibody [E178]; Ab32057, at a dilution of 1/500, staining Tau in paraffin embedded human brain sections by Immunohistochemistry.