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Anti-PRKAR2A Antibody E151
Also for PRKAR2A (NM_004157)
|A synthetic phospho-peptide corresponding to residues surrounding Ser96 of human PKA RII was used as immunogen. The antibody only detects PKA RII phosphorylated on Serine 96.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:2,500; IHC: 1:250; ICC: 1:500; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A)|
|PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3). |
TGF Beta Signaling Pathway
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Western blot - Anti-PKA R2 (phospho S99) antibody [E151]; All lanes : Anti-PKA R2 (phospho S99) antibody [E151] at 1/2500 dilution.Lane 1 : K562 cell lysate, untreated.Lane 2 : K562 cell lysate, treated with Alkaline Phosphatase.Predicted band size : 45 kDa.Observed band size : 51 kDa .
Western blot - Anti-PKA R2 (phospho S99) antibody [E151]; Anti-PKA R2 (phospho S99) antibody [E151] at 1/2000 dilution + Whole cell lystate prepared from human Vero cells at 20000 cells.Secondary.HRP conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution.Performed under reducing conditions.Predicted band size : 45 kDa.Observed band size : 51 kDa .Exposure time : 4 minutesThis image is a courtesy of Anonymous Abreview
Immunohistochemistry (Paraffin-embedded sections) - Anti-PKA R2 (phospho S99) antibody [E151]; ab32390, at a 1/250 dilution, staining (A) untreated (B) Phosphatase treated lymph node metastatic by immunohistochemistry, Paraffin embedded tissue
Immunocytochemistry/ Immunofluorescence - Anti-PKA R2 (phospho S99) antibody [E151]; ab32390, at a 1/500 dilution, staining (A) untreated and (B) Alkaline Phosphatase treated Hela cells by Immunocytochemistry/ Immunofluorescense.