OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PRKAR2A antibodies

Anti-PRKAR2A Antibody E151

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300306
  • Rabbit Monoclonal Antibody against PRKAR2A (Clone E151)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401337) , 20ug
100ul $325 3-7 Days
Add to Shopping Cart
WB(2)
IHC(1)
IF(1)
spacer
Also for PRKAR2A (NM_004157)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser96 of human PKA RII was used as immunogen. The antibody only detects PKA RII phosphorylated on Serine 96.
Clone NameE151 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2,500; IHC: 1:250; ICC: 1:500; IP: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A)
Alternative NamePKR2; PRKAR2
Database LinkNP_004148
FunctionPKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).
Related Pathway
TGF Beta Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-PKA R2 (phospho S99) antibody [E151]; All lanes : Anti-PKA R2 (phospho S99) antibody [E151] at 1/2500 dilution.Lane 1 : K562 cell lysate, untreated.Lane 2 : K562 cell lysate, treated with Alkaline Phosphatase.Predicted band size : 45 kDa.Observed band size : 51 kDa .
WB Image
Western blot - Anti-PKA R2 (phospho S99) antibody [E151]; Anti-PKA R2 (phospho S99) antibody [E151] at 1/2000 dilution + Whole cell lystate prepared from human Vero cells at 20000 cells.Secondary.HRP conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution.Performed under reducing conditions.Predicted band size : 45 kDa.Observed band size : 51 kDa .Exposure time : 4 minutesThis image is a courtesy of Anonymous Abreview
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Anti-PKA R2 (phospho S99) antibody [E151]; TA300306, at a 1/250 dilution, staining (A) untreated (B) Phosphatase treated lymph node metastatic by immunohistochemistry, Paraffin embedded tissue
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-PKA R2 (phospho S99) antibody [E151]; TA300306, at a 1/500 dilution, staining (A) untreated and (B) Alkaline Phosphatase treated Hela cells by Immunocytochemistry/ Immunofluorescense.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title