Home Antibody All anti-CCNE2 antibodies
Anti-CCNE2 Antibody E142
Also for CCNE2 (NM_057749)
|A synthetic peptide corresponding to residues in C-terminus of human Cyclin E2 was used as immunogen. Predicted to cross-react with mouse and bovine, based on sequence homology.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for IP.
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Cyclin E2 antibody [E142]; Anti-Cyclin E2 antibody [E142] at 1/500 dilution + HeLa cell lysate.Predicted band size : 45 kDa.Observed band size : 50 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Cyclin E2 antibody [E142]; Immunohistochemical analysis of Cyclin E2 expression in paraffin embedded human breast carcinoma using 1/50 TA300304.
Immunocytochemistry/ Immunofluorescence - Cyclin E2 antibody [E142]; Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 TA300304.
Flow Cytometry-Anti-Cyclin E2 antibody [E142](TA300304); Overlay histogram showing HeLa cells stained with TA300304 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.